GPT/ALT UV LIQUID

PRINCIPLE

The glutamic-pyruvate transaminase (GPT), actually ALT, catalyzes the reaction between L-alanine and α-ketoglutaric acid. The pyruvic acid formed is reduced by NADH with the aid of auxiliary enzyme LDH. The change of NADH to NAD+ produces a change of Abs. In optimum reaction conditions the ΔAbs/min is directly related to GPT concentration in the sample.

DIAGNOSTIC USE

High values of GPT activity are found in hepatocyte necrosis, cirrhosis or obstructive jaundice. The increase of GPT enzymatic activity is more specifi c for liver damage than ratio GOT/GPT. In viral hepatitis GPT increase is always greater than that of GOT. Also, higher values found in myocarditis, myocardial infarction or hemolytic diseases.Single test result could not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.