GOT/AST UV LIQUID, 50 mL

    998003



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    Conformité européenne In vitro diagnostic product

    For in vitro determination of Aspartate aminotransferase GOT/AST in serum or plasma.

    State Liquid
    Storage temp. +2 / +8 ºC
    Technique IFCC method

    PRINCIPLE

    The glutamic-oxalacetic transaminase (GOT), actually AST, catalyzes the reaction between L-aspartic acid and α-ketoglutaric acid. The oxalacetic acid formed is reduced by NADH with the aid of auxiliary enzyme MDH. The change of NADH to NAD+ produces a change of Abs. The reaction media contains, also, LDH to remove endogenous pyruvate to avoid possible interferences.In optimum reaction conditions the ΔAbs/min is directly related to GOT concentration in the sample.

    DIAGNOSTIC USE

    Increases in GOT activity were observed in cases of liver damage: hepatitis of various types, necrosis or damage in hepatocytes, cholestatic icteria.

    High levels are also seen in diseases aff ecting the heart muscle. In alcoholic hepatitis and acute myocardial infarction, the increase of GOT activity is greater than the activity of GPT.

    Single test result can not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.