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For in vitro diagnostic
PRINCIPLE
The stains which make up the Quick Panoptic stain combine polychromy and the quality of classic haematology staining methods (May-Grünwald, Giemsa, Wright) with a very quick execution time of just 15 seconds. The technique is performed by immersion in the staining solutions. As with the other Romanowsky stains, the basic dyes bind to the acidic components of cells, nucleic acids, granules in neutrophils and acidic proteins which are stained a relatively deep red-purple colour, while the acid dyes bind to haemoglobin, basic components of cell structures and eosinophil granules. Its use allows the differential staining of blood cells. The result of the staining may be infl uenced by several factors, such as the fi xation, the staining time and the pH value of the rinsing water. If the pH is too basic the staining will be more blue, and if the pH is too acidic, the staining will be more pink.
DIAGNOSTIC USE
For the differential staining of peripheral blood and bone marrow smears. It can also be used for the staining of semen samples.