SUDAN BLACK, 2 x 100 mL

PRINCIPLE

Sudan Black B is a diazo-series dye that stains various lipids, including neutral fats, sterols, and especially phospholipids. Formaldehyde vapors (fi xative) fi x fats and phospholipids which, in contact with the dye, take on a blue-black color that contrasts with the Wright dye used in counterstaining. The dye is insoluble in an aqueous cell medium but is solubilized in the lipid medium of certain granules, which stain a dark green, almost black.The black lipid granules appear on all granulocytes, intensifying as they change from myeloblasts to mature granulocytes. Lipid granules are also present in Auer bodies and in monocytes, in which they appear like a thin cytoplasmic layer. Lymphocytes, lymphoblasts and erythroblasts are negative to Sudan Black. It is used in haematology for diagnosing leukaemia, as it enables diff erentiation between monocytic and granulocytic leukaemia.

DIAGNOSTIC USE

For staining lipids in blood, bone marrow and tissue samples.