IRON - FERROZINE, 2 x 100 mL
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For in vitro determination of iron in serum or plasma.
State | Liquid |
Storage temp. | +2 / +8 ºC |
Technique | Colorimetric method |
PRINCIPLE
In a slightly acid medium and in the presence of guanidine hydrochloride, iron is released from transferrin and reduced by hydroxylamine from Fe (III) to Fe(II).
Divalent iron forms a coloured complex with FerroZine®, quantifiable spectrophotometrically.
(FerroZine®: Hach Chemical Co., Ames, IOWA, U.S.A)
DIAGNOSTIC USE
Iron is distributed in the body forming part of hemoglobin and myoglobin. Low iron concentations are found in infectious diseases and anemia. High levels of iron are presented in liver disease, hemochromatosis, and at high levels of Transferrin.
Single test result could not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.