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For in vitro determination of HbA1c in blood.
| State | Liquid |
| Storage temp. | +2 / +8 ºC |
| Technique | Immunoturbidimetric method |
PRINCIPLE
Total hemoglobin and HbA1c have the same unspecific absorption rate to latex particles. When mouse anti-human HbA1c monoclonal antibody is added, a latex-HbA1c-mouse ant-ihuman HbA1c antibody complex is formed. Agglutination is formed when goat anti-IgG polyclonal antibody interacts with the monoclonal antibody. The extent of agglutination is proportional to the amount of HbA1c absorbed on the surface of the latex particles. The method is based on latex – enhanced immunoturbidimetric analysis. Competitive binding is included in a first reaction step that allows the %HbA1c to be directly determined from the turbidity that develops during the second reaction.
DIAGNOSTIC USE
The HbA1c determination is useful in monitoring and control of diabetic patients. The concentration of this red blood cell protein is conditioned by the mean blood glucose level, for a period of 4-8 weeks, for what it turns out as a test not influenced by occasional sudden fluctuations in the serum glucose concentration. A single test result could not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.