URIC ACID LIQUID, 1 x 100 mL
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For in vitro determination of Uric acid in serum, plasma or urine.
State | Liquid |
Storage temp. | +2 / +8 ºC |
Technique | Uricase - POD method |
PRINCIPLE
The uric acid of the sample is degraded by the action of uricase to allantoin with the release of hydrogen peroxide. Quantification of hydrogen peroxide released is performed by Trinder reaction, wherein a coloured quinone compound is formed by reacting with 4-aminoantipyrine and the chromogen 3,5-dichloro-2-hydroxysulfonate in the presence of peroxidase (POD). The colour produced in the reaction is proportional to the concentration of uric acid of the sample under optimal assay conditions.
DIAGNOSTIC USE
Elevated uric acid values are found in kidney disease, gout, hyperthyroidism, malignancies and myeloproliferative diseases. Lower values than those usual ones are found in congenital disorders of metabolism as xanthinuria and in cases of poor purine intake.
The usual range of values for healthy subjects is large, depending on the feed, age, sex, and also, it seems to be related to inherited genetic variations. A single test result cannot be used to make a clinical diagnosis. It should integrate clinical and laboratory data.